Composite

Part:BBa_K4166000:Design

Designed by: Alexa DeKorte, Christina Harbin   Group: iGEM22_Alma   (2022-08-30)


Quorum sensing kill-switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 348
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MazF cuts at ACA sequences, which can be included or excluded to effect latency of inhibition


Source

Generated from DNA in registry, synthesized by IDT

References